Wenteng Liu

Single-cell long-read transcriptomics: from technologies to biological insights

This review examines the experimental and computational foundations of scLR-seq, including platform selection, library design, cell barcode and unique molecular identifier recovery, transcript discovery, and isoform quantification, and summarize emerging insights into isoform usage, alternative splicing, transcription start and end site selection, allele-specific expression, fusion transcripts, transposable element-derived transcripts, and RNA modifications.