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Author

Xiang-Hui Qi

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Aug 2026

Biosensor-driven Evolution and Transcriptome-Guided Chassis Engineering Enhance Endogenous l-Cysteine Supply for Ergothioneine Production.

An Escherichia coli platform for glucose-derived EGT production was developed by combining biosensor-driven evolution and transcriptome-guided chassis engineering, enabling sequential growth-based enrichment and fluorescence-based prioritization.

Su-Yu Wang, H. Zabed, Guoyan Zhang et al. · 0 citations
Aug 2026

Combinatorial Lid-Domain Engineering and Computational Design Enable Simultaneous Enhancement of Activity and Thermostability of D-Allulose 3-Epimerase.

d-Allulose is a promising low-calorie rare sweetener; however, its industrial production is limited by the insufficient catalytic activity and thermostability of d-allulose 3-epimerases (DAEases). In this study, a combinatorial engineering strategy integrating lid-domain modulation and computational design was develope...

Qi-Chen Liu, Xin-Rui Tang, Wen-Jing Sun et al. · 0 citations
Jul 2026

Periplasmic Engineering Enhances Terminal Hydroxylation for Efficient Caffeic Acid Biosynthesis in Escherichia coli.

Caffeic acid (CA) is a valuable phenylpropanoid with applications in food, pharmaceutical, and chemical industries. Microbial production of CA is often limited by the terminal hydroxylation catalyzed by 4-hydroxyphenylacetate 3-monooxygenase (HpaBC). Here, we constructed a de novo CA biosynthetic pathway in Escherichia...

Shangyi Wang, Yuqi Zhuo, Jamila A Tuly et al. · 0 citations
Aug 2026

Parallel Multidimensional Engineering of Acetylxylan Esterase Enables Concurrent Enhancement of Thermostability and Catalytic Efficiency.

Simultaneously enhancing the thermostability and catalytic activity of acetylxylan esterases (AxEs) remains a significant challenge due to the inherent stability-activity trade-off. Here, a previously uncharacterized CE7 family acetylxylan esterase (TsAxE) from Thermoclostridium stercorarium was identified and engineer...

Yu-Jiao Tao, Xin-Rui Tang, Mei Zhao et al. · 0 citations
Open access Jul 2026

Multi-level precise regulation of gene transcription in the yeast Saccharomyces cerevisiae based on light-sensitive CRISPR/Cas systems

The gene regulatory approaches at the translational and post-translational levels were integrated with optogenetic control patterns to attain very sensitive multi-level precision regulation of the dCas9 protein, thereby facilitating flexible regulation of transcription levels of target genes.

Yaokun Liang, Xianghui Qi, Song Gao et al. · 0 citations

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