Analysis of N-Glycans Released from Glycosylated RNAs.
Glycosylated RNAs (glycoRNAs) are involved in immune response, cellular function, gene regulation, and disease development. We report a liquid chromatography-mass spectrometry (LC-MS) method to analyze glycans released from glycoRNAs. The developed method includes extracting RNA from mouse liver using TRIzol, digesting...