Xiao-Lin You

An integrated engineering strategy enhances expression and activity of Pseudonocardia thermophila nitrile hydratase in Escherichia coli.

Nitrile hydratase is a key enzyme for nitrile-to-amide hydration under mild conditions, yet its application is limited by low heterologous expression, poor solubility, and suboptimal catalysis. Here, we present an integrated strategy to enhance expression, assembly, and function of Pseudonocardia thermophila NHase (PtN...