Author

Xiao-Nan Yang

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Sep 2026

CHAI-sEV: A Programmable Hairpin-Driven Cas9 Platform with AI Integration for Dual-Protein Profiling and Intelligent Classification of Tumor-Derived Small Extracellular Vesicles

Multiplexed protein profiling of tumor-derived small extracellular vesicles (TsEVs) requires signal-conversion strategies that are sensitive, low-background, and orthogonal. Collateral-cleavage CRISPR assays based on Cas12a or Cas13a provide efficient amplification but can compromise single-pot multitarget detection,...

Hao-Yu Shen, Xin-Yuan Pan, Yan Wu et al. · Analytical Chemistry · 0 citations

Aug 2026

Orthogonal Cas13a/Cas12a cascade for one-pot amplification-free detection of miRNA-21.

MicroRNAs (miRNAs) are promising biomarkers for clinical diagnosis and disease monitoring. However, current CRISPR/Cas-based miRNA sensors generally require reverse transcription or nucleic acid amplification to improve sensitivity, which complicates the workflow and increases the risk of contamination, nonspecific amp...

Jing Yuan, Meng Shen, Li-Hua Ding et al. · Talanta: The International J... · 0 citations