Rapid Detection of Spring Viremia of Carp Virus (SVCV) Using RAA-CRISPR/Cas12a.
A recombinase-mediated isothermal amplification (RAA) in conjunction with the CRISPR/Cas12a system targeting the screened conserved region of the G gene in the genome to identify SVCV is established, offering a diagnosis project in resource-limited aquaculture environments and a field-applicable early warning of SVCV.