Construction of an infectious clone of tomato leaf curl New Delhi virus and development of a duplex assay through recombinase polymerase amplification-lateral flow dipstick to detect the virus and tomato yellow leaf curl virus simultaneously
The co-infection of tomato yellow leaf curl virus (TYLCV) and tomato leaf curl New Delhi virus (ToLCNDV) in the same host complicates disease diagnosis and management, not to mention that each virus alone can cause devastating losses to its respective host crops across many tomato- and cucurbit-growing areas. However...