De Novo Biosynthesis of cis -1,4-Polyisoprene from Methanol in Pichia pastoris via Systematic Metabolic Engineering
cis-1,4-Polyisoprene, the primary component of natural rubber, is a critical industrial polymer traditionally sourced from Hevea brasiliensis or petroleum. Microbial production offers a sustainable alternative, but achieving efficient synthesis and high molecular weight remains challenging. Here, we report the de nov...