CRISPR/Cas9-Mediated Knockout of the Chorion Gene s36 Impairs Ovarian Development and Abolishes Egg Hatching in the Melon Fly Zeugodacus cucurbitae
The melon fly, Zeugodacus cucurbitae (Coquillett), is a globally invasive pest of cucurbit crops whose concealed larval feeding limits chemical control and has driven interest in reproduction-based management. The chorion gene s36 encodes an eggshell protein conserved among dipterans. However, whether s36 is required f...