Characterization of two key cytochrome P450 enzymes in Isodon amethystoides and de novo production of 3α,15β-dihydroxy-ent-kaurene in yeast
This work identified two enzymes, IamCYP71D761 and IamCYP706V18, in Isodon amethystoides, which specifically mediate the C3α- and C15β-hydroxylation of ent-kaurene with high stereoselectivity and regioselectivity, respectively, generating 3, a key precursor in the biosynthesis of GLA.