Plasma membrane intrinsic proteins (PIPs) are an important subfamily of aquaporins that function as membrane channels that facilitate water transport across the plasma membrane in plants. Although the molecular mechanisms of PIPs have been extensively studied in model species, their characteristics and functions remain poorly characterized in
Caragana korshinskii
. Here, 13
CkPIP
genes were identified through genome-wide analysis. CkPIPs were clustered into PIP1 (seven members) and PIP2 (six members) subfamilies. They exhibited conserved physicochemical properties, contained cis-acting elements responsive to abiotic stresses and phytohormones, and showed evidence of segmental duplication under purifying selection. Phylogenetic, structural, and expression analyses (RNA-seq and RT-qPCR) were performed to explore their evolutionary patterns and responses to drought stress. Under drought stress,
CkPIPs
exhibited tissue-specific expression dynamics:
CkPIP1;1
,
CkPIP1;2
, and
CkPIP2;2
were prominently upregulated, indicating that they play important roles in drought adaptation. This study expands our understanding of the characteristics of the CkPIP family and provides valuable molecular insights for drought-resistance research and forage improvement.
Wenyu Li, Hatieti Madeti, Xinyi Liu et al.· BMC Plant Biology· 0 citations
Selinexor (KPT-330), the first oral selective nuclear export inhibitor, simultaneously modulates key signaling pathways, including NF-κB, JAK/STAT, FOXO, Nrf2, and NLRP3, by blocking XPO1-mediated nuclear export, thereby offering a novel multi-target strategy for treating chronic inflammatory diseases. This review systematically integrates existing preclinical and early clinical evidence within the framework of "cytokine signaling networks", focusing on elucidating the molecular mechanisms and biological effects of selinexor in suppressing proinflammatory factor production, mitigating oxidative stress, and regulating inflammatory tissue-remodeling networks. Recent findings further indicate that SINE compounds can remodel proteostasis, including ankyrin repeat and SOCS box-containing protein 8 (ASB8)/Cullin-RING ligase 5 (CRL5)-associated XPO1 degradation and regulation of the ACE2-TMPRSS2-XPO1 coronavirus-entry network. However, current evidence primarily stems from in vitro and animal studies, and randomized controlled trials in human chronic inflammatory diseases are lacking. Moreover, hematopoietic and gastrointestinal toxicities observed in oncology settings suggest a narrow therapeutic window. This review emphasizes a stepwise translational logic from protein turnover and receptor regulation to next-generation XPO1 inhibitors. At present, selinexor is more suitable as a mechanistic tool for exploring the XPO1-inflammation axis, whereas inflammatory-disease translation will require eltanexor or related agents with more favorable tissue distribution and tolerability, together with precisely stratified clinical studies.
Hai Zhang, Xinyi Liu, Yunhua Zhao et al.· International Immunopharmaco...· 0 citations
