Structure, stability, and antioxidative mechanism of novel antioxidant peptides from Agaricus bisporus prepared by three-step purification.
Three novel antioxidant peptides (FPEF, LGFD, MGFGL, molecular weight <1000 Da) were isolated from Agaricus bisporus via sequential DA201-C macroporous resin, Sephadex G-100 gel filtration, and RP-HPLC. Molecular docking revealed strong Keap1 binding (energies: -8.2 to -8.7 kcal/mol) via hydrogen bonds and π-alkyl inte...