Mutations at the glucose-stimulation site in the gatekeeper region of β-glucosidase CbiCba3 from Cellulomonas biazote reveal an activity-stability trade-off.
β-Glucosidase (BGL) catalyzes the hydrolysis of cellooligosaccharides into glucose during lignocellulosic biomass saccharification and is widely used in industrial applications such as bioethanol and biorefinery processes, food and beverage production, and platform chemical manufacturing. The BGL CbiCba3 exhibits gluco...