Y. Sterkers

Comparative study of a reference PCR assay targeting kinetoplast DNA and a commercial kit targeting ribosomal DNA for the molecular diagnosis of visceral leishmaniasis

ABSTRACT Visceral leishmaniasis (VL) is a severe parasitic disease that requires accurate and timely diagnosis to prevent fatal outcomes. Molecular methods, particularly quantitative PCR (qPCR), have become central to VL diagnosis because of their high sensitivity and specificity. However, assay performance depends on...