Systematic identification of permissive insertion sites for foreign gene expression in a NADC30-like PRRSV infectious clone and development of dual-fluorescent recombinant viruses.
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen threatening the global swine industry. Infectious clone and recombinant virus platforms serve as vital tools for investigating PRRSV biology and developing genetically engineered vaccines. In this study, we constructed a full-length infecti...