Author

Yan Chen

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Open access Aug 2026

Adaptive laboratory evolution rewires Pseudomonas putida for resource-efficient acetate assimilation

Acetate is an attractive renewable two-carbon substrate for microbial biotechnology, but its toxicity limits growth and carbon-use efficiency at process-relevant concentrations. Here, we used adaptive laboratory evolution to improve acetate tolerance in a genome-reduced strain of Pseudomonas putida and combined whole-g...

N. Gurdo, A. Srinivasan, Tommaso Tagliani et al. · bioRxiv · 0 citations

Open access Jul 2026

Simultaneous optimization of lignocellulosic sugar catabolism via systematic laboratory evolution under complex selection pressure

Efficient co-utilization of hexose and pentose sugars from lignocellulose is essential for microbial bioconversion, yet engineered catabolic pathways can be unstable or suboptimal in complex resource environments. Here, we use a Pseudomonas putida strain engineered to catabolize xylose and arabinose to examine how reso...

Sunghwa Woo, H. Lim, B. Norton-Baker et al. · Nature Communications · 0 citations