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Author

Yan-Qian Li

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Open access Oct 2026

A direct RNA sequencing workflow for simultaneous profiling of tRNA abundance and modifications in mouse liver

Transfer RNA (tRNA) is highly modified and difficult to profile using reverse-transcription-based sequencing. Here, we established a direct RNA sequencing (tRNA-DRS) workflow to simultaneously characterize tRNA abundance and modification signals in mouse liver. The workflow included small-RNA enrichment, deacylation, s...

Sheng-Hong Dai, Zhao-Yang Tian, Ya-Xuan Zhang et al. · 0 citations
Open access Oct 2026

scTHREAD: a cell-resolved long-read transcriptome database with linked isoform, poly(A), junction, and allelic evidence.

Cell-resolved transcript structures are essential for understanding how isoform usage, alternative polyadenylation, and allelic expression vary across cell types. However, existing single-cell long-read datasets remain fragmented across studies, platforms, species, and workflows, hindering comparison and reuse. Here, w...

Zhi-Can Fu, Ye-Lian Yan, Chun-Chun Wu et al. · 0 citations

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