Engineering a thermostable MHET-active esterase through active-site compaction for efficient dual-enzyme PET depolymerization.
Efficient enzymatic recycling of polyethylene terephthalate (PET) is frequently hindered by the accumulation of mono-(2-hydroxyethyl) terephthalate (MHET), a soluble intermediate that competitively suppresses PET hydrolase activity. Although MHET-hydrolysing enzymes can overcome this limitation, these enzymes often exh...