Function-Guided Tunnel Remodeling Enables Regioselective Synthesis of Hydroxysalidroside via Engineered Microbial UDP-Glycosyltransferase with Record Productivity
Regioselective glycosylation of polyhydroxylated natural products remains a major bottleneck for deploying glycosyltransferases in sustainable biomanufacturing. Herein, we developed a function-guided substrate tunnel remodeling strategy to redesign a microbial UDP-glycosyltransferase from Bacillus paralicheniformis (...