How to Mitigate CRISPR-Cas9 Off-Target Effects: Evaluation of Current Detection, Prediction, and Mitigation Strategies
This review provides a thorough overview of the current state of knowledge on off-target causes, detection and prediction methods, and mitigation strategies for CRISPR-Cas9 applications, and integrates these dimensions to provide a clear framework for controlling when, where and how CRISPR-Cas9-mediated alterations occur.