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Open access Aug 2026

Leveraging distributed acoustic sensing for large-scale expressway traffic state perception

This paper introduces distributed acoustic sensing (DAS) as an emerging sensing technique for large-scale traffic state perception (TSP) on expressways. By enabling optical fibers to operate as dense sensing arrays, DAS offers a promising solution for continuous traffic monitoring along expressway corridors. However, the raw traffic information extracted from DAS is inaccurate, which limits its direct application to large-scale TSP. This study proposes a physics-informed neural network (PINN) framework for DAS-based TSP. A physics-based DAS simulation platform is developed and validated against field observations which provides a flexible testbed for generating data under diverse traffic scenarios. On this basis, two network architectures, ResUNet and Fourier Neural Operator, are investigated in combination with three types of traffic flow constraints, namely the Lighthill-Whitham-Richards (LWR) model, LWR with a fundamental diagram, and the Aw-Rascle-Zhang model. Totally, 16 PINN models are constructed and evaluated. The results show that the proposed PINN framework effectively improves the accuracy of DAS-based TSP compared to the solely data-driven baselines. Among the physical constraints considered, the LWR-based models show the best overall performance. In addition, few-shot transfer learning can enhance model performance in previously unseen scenarios, demonstrating the potential of the proposed framework for adaptation to site-specific deployment conditions.

Yang Ma, Dianwei Zhou, Yang Liu et al. · 0 citations
Open access Jul 2026

A decoupled transcription platform enables tunable and predictable gene expression in yeast

Predictable control of gene expression is essential for building genetic circuits and improving metabolic pathways, but conventional promoter libraries often behave unpredictably when genes are combined. Here we develop CRISPR-Activated Promoter-based Orthogonal expression (CAPO), a quantitative platform for controlling multiple genes in yeast. CAPO uses synthetic CRISPR-activated promoters that remain silent until matching guide RNAs recruit dCas9-VPR. We tune each gene by varying guide RNA abundance with defined T7 promoters, while keeping regulatory channels orthogonal. CAPO reaches expression levels comparable to strong native yeast promoters, maintains low background activity, and preserves promoter-strength order across different genes. We apply CAPO to program broad fluorescence color outputs and to rapidly optimize lycopene and 3-hydroxypropionic acid biosynthesis. These results establish CAPO as a scalable platform for predictable engineering of eukaryotic gene networks. Efficient bioproduction using eukaryotes, such as engineered Saccharomyces cerevisiae, requires precise control over gene expression. Here, authors develop CAPO, a CRISPR-guided system that tunes gene activity in yeast and enables multiplex colour generation and faster optimization of metabolic pathways.

Yu-Jie Chen, Hui Li, Lingling Duan et al. · 0 citations