N6-methyladenosine (m6A) RNA modification regulates pulmonary inflammation, yet the precise function of the m6A reader IGF2BP2 in acute lung injury (ALI) remains elusive. Here, we reveal that IGF2BP2 and global m6A levels are significantly upregulated in lipopolysaccharide (LPS)-induced ALI. Prophylactic genetic silencing or pharmacological inhibition of IGF2BP2 robustly mitigated pulmonary hyperinflammation, epithelial apoptosis, and pathological tissue damage. Mechanistically, transcriptome profiling identified MARCKSL1 as a direct downstream target. We demonstrate that IGF2BP2 binds and post-transcriptionally stabilizes MARCKSL1 mRNA in a partially METTL3/m6A-dependent manner. Elevated MARCKSL1 subsequently drives NF-κB p65 nuclear translocation and activation, fueling the inflammatory cascade. Strikingly, we trace this IGF2BP2 dysregulation upstream to inflammatory metabolic reprogramming: elevated alveolar glycolysis drives lactate accumulation, which triggers EP300-mediated histone lactylation (H3K18la) at the IGF2BP2 promoter to activate its transcription. Pharmacological manipulation of glycolysis confirmed this metabolic-epigenetic coupling. Collectively, our findings decipher a novel H3K18la/IGF2BP2/MARCKSL1 signaling cascade that integrates metabolic reprogramming with m6A epitranscriptomics in ALI, highlighting IGF2BP2 as a promising target for preventive intervention in airway epithelial inflammation.
Yangdi Peng, Junyao Zhang, Yang Yang et al.· Molecular Immunology· 0 citations
BACKGROUND
Missed nursing care (MNC) in intensive care units (ICUs) is associated with adverse patient outcomes, yet multicentre evidence from mainland China remains limited.
AIM
To describe the level and pattern of MNC among ICU nurses in China and to identify associated factors.
STUDY DESIGN
Multicentre cross-sectional survey across China.
RESULTS
Of the 900 questionnaires distributed, 840 valid responses were included in the final analysis. The mean total MNC score was 98.9 ± 22.4. Psychological support, basic nursing care and rehabilitation care were the most frequently missed domains. The mean score for perceived causes of MNC was 72.3 ± 15.8, with absolute and relative shortages of human resources and communication-related factors identified as the leading contributors. Average number of patients cared for per shift, ICU specialist nurse qualification, professional title and hospital level were significantly associated with total MNC score, together explaining 24.4% of the variance.
CONCLUSIONS
MNC is common in Chinese ICUs, particularly in psychosocial, basic and rehabilitation-related care. Organisational context, nurse workload and professional preparation appear to be important factors associated with MNC. Targeted strategies are needed to optimise staffing, strengthen specialist training and improve communication processes in ICU teams.
RELEVANCE TO CLINICAL PRACTICE
Better understanding of the pattern and key associated factors of MNC may help nurse managers prioritise staffing, competency development and communication improvement to enhance ICU nursing quality and patient safety.
Su-Jun Xiao, Rong Jiang, Qin Xiong et al.· Nursing Critical Care· 0 citations
OPD functions as a novel molecular degrader of KIF11, exerting its anti-LUAD effects by promoting KIF11 ubiquitination and subsequent proteasomal degradation.