Overcoming Steric Hindrance for Precise Multiplexed Protein Profiling of Single Extracellular Vesicles.
Multiplexed protein profiling of single extracellular vesicles (EVs) is crucial for precise liquid biopsy but is severely limited by steric hindrance arising from bulky detection probes. This study systematically deconstructs the sources and consequences of this limitation using high-sensitivity nano-flow cytometry (nF...