IL-10 promoter polymorphisms (-1082G > A,-819C > Tand - 592 C > A) and pediatric asthma susceptibility in a Mauritanian population.
BACKGROUND Interleukin-10 (IL-10) is a key immunoregulatory cytokine implicated in asthma pathogenesis. This case-control study investigated the association of three IL-10 promoter polymorphisms (-1082 G > A, -819 C > T, and -592 C > A) with asthma susceptibility and immune cell profiles in a Mauritanian pediatric population. METHODS Fifty-four asthmatic children and 45 controls were genotyped by PCR-RFLP and Sanger sequencing. Allele/genotype frequencies were compared by chi-square test, haplotypes by Haploview, and IgE, leukocyte, and platelet counts were analyzed using non-parametric tests. RESULTS The -592 C > A polymorphism was significantly associated with asthma, with the A allele more frequent in cases and the CA genotype increasing risk. The -1082 G > A polymorphism showed a significant genotype association, with the GA genotype protective and the AA genotype observed only in cases. The ACG haplotype showed a trend toward increased risk.Asthmatic children exhibited higher IgE levels, neutrophil and basophil percentages, and lower lymphocyte, monocyte, and platelet counts. The -1082 G > A variant was associated with neutrophils and monocytes in the overall population. Stratified analysis revealed disease-specific effects: -819 C > T influenced monocytes in cases, while -592 C > A affected eosinophils in controls. CONCLUSION IL-10 promoter polymorphisms, particularly -592 C > A, are significantly associated with pediatric asthma susceptibility, with -1082 G > A showing an additional protective effect. Asthmatic children display a mixed inflammatory profile of elevated neutrophils and basophils alongside reduced lymphocytes and monocytes. The -1082 G > A, -819 C > T, and -592 C > A variants show immunomodulatory effects on leukocyte composition in a disease-context-dependent manner. Replication in larger cohorts with functional IL-10 measurements is warranted.