Multifaceted Cross-Rational Modification of the α2,3-Sialyltransferase MsST for High-Efficiency Production of 3'-Sialyl-3-fucosyllactose via One-Pot Cascade Reaction.
3'-Sialyl-3-fucosyllactose (3'S3FL) is a representative fucose-containing sialylated human milk oligosaccharide with distinctive biological functions, but its in vitro synthesis is limited by the poor tolerance of α2,3-sialyltransferases toward fucosylated acceptors. Here, we report an engineered α2,3-sialyltransferase...