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Yi-Fan Cui

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Open access Sep 2026

Establishment of a genome editing system and expression of heterologous proteins in the nonconventional yeast Pichia kudriavzevii

This work established a highly efficient CRISPR/Cas9 genome editing system in this diploid yeast, enabling marker‐free editing with efficiencies of up to 88% and routine generation of homozygous mutants and successfully expressed secretory mCherry and human IgG1 Fc fragment at different loci.

Jia-Yi Ma, Yi-Fan Cui, Bin-Ru Yin et al. · 0 citations

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