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These findings establish antibiotic-driven phase separation as a previously unrecognized mechanism to encode cellular signaling and identify antibiotic condensates as a distinct functional unit underlying drug tolerance.
Biomolecular condensates, which regulate diverse cellular processes, exhibit distinct electric potential profiles. This potential gradient between the dilute and the dense phases serves as the underlying driving force mediating the unique microenvironment and electrochemical activity of condensates. However, the molecu...
This chapter describes synthetic gene circuit-based methods for applying synthetic condensates in E. coli to regulate transcription and translation and demonstrates how engineering synthetic condensates offers a new layer of cellular control for synthetic biology.
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