Author

Yifan Wang

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Open access Jul 2026

Saturated mutagenesis screen of M-MLV reverse transcriptase identifies variants enhancing prime editing efficiency

A compact prime editor is developed by deleting the RNase H domain of the MMLV reverse transcriptase and a saturated mutagenesis screen targeting two DNA interacting regions within the PE2ΔR-RT Fingers domain is conducted to identify novel mutations that enhance prime editor performance.

Hui Li, Yifan Wang, Chenxin Zhang et al. · bioRxiv · 0 citations