Combining network toxicology, single-cell RNA sequencing, and in vitro/in vivo experimental models reveals MMP9 as the key mediator of 27-hydroxycholesterol-driven ovarian cancer.
27-Hydroxycholesterol (27-HC), as a major cholesterol metabolite, has been shown to be associated with tumor biology, but its role in ovarian cancer remains unclear. This study integrates network toxicology, multi-cohort transcriptomic validation, and single-cell RNA sequencing to systematically investigate the potenti...