Vanillin is a widely used flavor compound in the food, cosmetic, and pharmaceutical industries. Although microbial biosynthesis offers a green alternative to conventional chemical synthesis, challenges such as unstable heterologous enzyme expression, insufficient methyl donor supply, and product cytotoxicity limit de n...
Ming-Yue Tian, Jin-Yi Li, Yong Du et al.· Synthetic and Systems Biotec...· 0 citations
Vanillyl alcohol (VNA) is a high-value compound used in the pharmaceutical industry and other fields. Its microbial de novo biosynthesis is severely limited by the low catalytic efficiency of O-methyltransferase. Here, we constructed a V314I mutant of caffeate O-methyltransferase via structure-guided protein engineer...
Jin-Yi Li, Yong Du, Jia Wang et al.· ACS Synthetic Biology· 0 citations
Daphnetin is a clinically established coumarin, but its native biosynthetic pathway remains elusive. In this study, a 2-oxoglutarate-dependent dioxygenase (2OGD), AtS8H, was identified that hydroxylates umbelliferone (UMB) to daphnetin , enabling the design of an artificial biosynthetic pathway. However, the pathway ef...
Shunmin Ji, Chong Xie, Yanyan Wang et al.· ACS Synthetic Biology· 0 citations
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