An integrated strategy of multiplex qPCR and BclA gene length polymorphism analysis for differentiating Bacillus anthracis wild-type and vaccine strains
ABSTRACT A combined molecular strategy was developed to accurately differentiate wild-type and vaccine strains of Bacillus anthracis. This approach integrates a multiplex TaqMan qPCR assay targeting the BclA, sap, and cap genes with conventional PCR analysis of BclA fragment length polymorphism. The qPCR assay demonstr...