Author

Yoshimitsu Kakuta

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Open access Oct 2026

Structural and functional analysis of KfiA, a UDP-GlcNAc transferase involved in Escherichia coli K5 heparosan biosynthesis.

Heparosan is a linear glycosaminoglycan-related polysaccharide produced as the capsular polysaccharide of Escherichia coli K5. Its biosynthesis requires alternating transfer of N-acetylglucosamine and glucuronic acid by the glycosyltransferases KfiA and KfiC, respectively, but the structural basis of KfiA function has...

Kento Maeda, Aya Sakamoto, Kyoka Matsuura et al. · Carbohydrate Research · 0 citations

Sep 2026

Biochemical analysis of catalytic and disease-associated residues in mouse Golgi-resident 3'-phosphoadenosine-5'-phosphate (PAP) phosphatase BPNT2/gPAPP.

Golgi-resident 3'-phosphoadenosine-5'-phosphate (PAP) phosphatase BPNT2/gPAPP plays an essential role in maintaining sulfation homeostasis, but its biochemical characterization has been limited by the difficulty of in vitro analyses. Here, we established a stable expression and activity assay system using a Trigger fac...

Ryota Urushihara, Yasuhisa Maruno, Saki Maruoka et al. · Bioscience, biotechnology an... · 0 citations

Open access Aug 2026

Hydration Energetics Shape Antibody Discrimination between Sulfotyrosine and Phosphotyrosine

Chemically similar post-translational modifications can mediate distinct biological functions, but how proteins distinguish between them remains unclear. Sulfotyrosine (sTyr) and phosphotyrosine (pTyr) exemplify this problem because they have similar sizes, local geometries, and electrostatic properties but function in...

Takahiro Mori, Kota Yahagi, Saki Maruoka et al. · bioRxiv · 0 citations