De novo chromosome-scale genome assembly of two mycoparasitic hypocreaceous fungi (Hypocreaceae, Ascomycota)
Mycoparasitic fungi in the Hypocreaceae family are ecologically and economically significant, yet genomic resources for many lineages remain limited. This is particularly true for Hypomyces and some related genera, which include causative agents of cobweb and web bubble diseases in commercial mushroom farms. Here, we present high-quality genome assemblies and annotations for two mycoparasitic Hypocreaceae fungi, Hypomyces semicircularis X01500 and Mycogone sp. X01501 representing a distinct phylogenetic lineage within Mycogone . Using a hybrid sequencing approach (Illumina and ONT), we generated chromosome-scale assemblies for X01501 (32,977,754 bp, 8 contigs, N50 = 4,917,145 bp) and X01500 (39,466,758 bp, 8 contigs, N50 = 5,660,398 bp), with BUSCO completeness > 99.7%. We annotated 9,717 and 11,121 protein-coding genes in genomes of X01501 and X01500, respectively, the majority of which received functional annotation. The metrics indicate high assembly continuity, completeness, and accuracy, with seven nuclear contigs and one mitochondrial contig recovered for each strain. These genomes provide a foundation for future research into the evolution and mechanisms of mycoparasitism within this understudied clade.