BioFlowBench: A Comprehensive Benchmark for Evaluating Bioinformatics Tool-use Capabilities of LLMs and Agents
The rapid advancement of high-throughput technologies has led to an explosion of biological data and a subsequent surge in bioinformatics analysis tools, thereby creating an urgent demand for automated bioinformatics workflows. Recently Large Language Models (LLMs) and LLM-based agents show great potential in this area. However, existing benchmarks primarily focus on static question-answering (QA) tasks, failing to capture the knowledge-action gap between understanding tool usage and executing complex bioinformatics workflows. Furthermore, current evaluation paradigms often prioritize algorithmic success rates, while neglecting the biological validity. Moreover, the construction of execution benchmarks is challenging due to complex environmental dependencies and the high cost of manual annotation, leading to poor scalability. In this study, we propose BioFlowBench, a comprehensive benchmark designed to shift from static knowledge assessment to dynamic execution evaluation in bioinformatics tool utilization. First, we construct a multi-layered dataset consisting of 5,071 test samples, including Syntax Understanding, Contextual Application and Real-world Execution. Second, we introduce BioGen, an agent-based pipeline designed for the automated generation of executable benchmarks. By creating compact, low-overhead synthetic data, BioGen facilitates low-cost and large-scale testing. Third, we propose a multi-dimensional evaluation framework comprising static knowledge, structural integrity, functional validity, and efficiency metrics. Our experiments reveal that: (1) A significant gap exists between static QA and dynamic execution tasks, with top LLMs perform well on static QA but falter in real-world execution scenario; (2) specialized agents outperform general models in real-world execution through environmental interaction and iterative refinement; and (3) domain knowledge remains the primary bottleneck, often leading to executable but biologically inaccurate outputs. The code is available at: https://github.com/YufeiHouAnne/BioFlowBench and the dataset can be accessed at: https://www.scidb.cn/detail?dataSetId=aee284681d674f53bfc6dae44635e773.