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Yuwei Ma

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Jul 2026

Rational Design of Flexible Loops Adjacent to the Active Site Enhances the Catalytic Efficiency of Bacillus β-Mannanase.

This work rationally engineered flexible loops near the active site via molecular dynamics, NMsim, NMsim, sequence conservation analysis, and MAESTRO prediction, and obtained the optimal double mutant M1, which showed 1.54-fold higher specific activity and nearly 2-fold improved pH stability at pH 8, with a nearly doubled kcat while retaining substrate affinity.

Yuan Li, Yuwei Ma, Tongli Li et al. · 0 citations