Author

Yuwen Tian

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Open access Jul 2026

Rational and computation-assisted engineering of a compact and efficient StaCas9 genome editor

Natural variation in PAM recognition among SaCas9 orthologs is analyzed and StaCas9 is identified as a compact and efficient nuclease recognizing an NNG PAM, establishing StaCas9 as a high-performance genome-editing tool for therapeutic applications.

Miaomiao Li, Wenni You, Yuwen Tian et al. · bioRxiv · 1 citation