Production of Hen Egg-White Lysozyme: Recombinant Expression in Pichia pastoris and Isolation of the Native Enzyme
Hen egg-white lysozyme (HEWL) is a well-characterized antimicrobial enzyme widely used in the food industry, biotechnology, and biomedical research. The aim of this study was to investigate two approaches to lysozyme production: heterologous expression of its gene in Pichia pastoris and isolation of native HEWL from ch...