Substrate Channeling on Bienzyme Assembly Significantly Enhances Cascade Biocatalysis of Ene Reductase and Aldehyde Dehydrogenase at High Substrate Concentrations
Multienzyme cascades enable efficient synthesis of value-added chemicals by coupling sequential biotransformations. In the ene reductase (OYE)–aldehyde dehydrogenase (ALDH) cascade for chiral α-substituted carboxylic acids, accumulation of the saturated aldehyde intermediate inhibits OYE and thereby limits cascade pe...