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Open access Aug 2026

TxNova: recovery of recurrent unannotated intergenic splice loci from existing bulk RNA-seq alignments

Background Reference catalogs such as GENCODE capture most stably expressed mammalian genes but may not include condition-restricted or low-abundance transcripts. Reads supporting unannotated intergenic splice junctions are generally absent from annotation-restricted gene-count matrices; transcript assemblers may recon...

Zhao Li, A. James, Sheng-Xuan Li · 0 citations
Review Open access Aug 2026

scATrans: annotating single-cell differential expression as transcription- or stabilization-weighted using unspliced RNA

What scATrans adds is the inference layer single-cell reanalysis actually needs—DE-defined membership, gene-structure correction, a capture-regime reliability pre-flight, induction-matched testing, and a permutation-calibrated program score—so that confident calls are reserved for where the data support them: gene prog...

Zhao Li, A. James, Sheng-Xuan Li · 0 citations

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