Background Reference catalogs such as GENCODE capture most stably expressed mammalian genes but may not include condition-restricted or low-abundance transcripts. Reads supporting unannotated intergenic splice junctions are generally absent from annotation-restricted gene-count matrices; transcript assemblers may recon...
Zhao Li, A. James, Sheng-Xuan Li· bioRxiv· 0 citations
What scATrans adds is the inference layer single-cell reanalysis actually needs—DE-defined membership, gene-structure correction, a capture-regime reliability pre-flight, induction-matched testing, and a permutation-calibrated program score—so that confident calls are reserved for where the data support them: gene prog...
Zhao Li, A. James, Sheng-Xuan Li· bioRxiv· 0 citations
A fixed budget near 2,000 HVGs is frequently suboptimal, and list cardinality is a separable design axis that can be automated without changing the ranking formula.
Zhao Li, A. James, Sheng-Xuan Li· bioRxiv· 1 citation
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