Detection of nucleic acids at the single-molecule level is a cornerstone of next-generation diagnostics. Unmodified glass nanopore-based sensing stands out among single-molecule detection techniques for its simplicity and versatility. This review presents the key advantages of unmodified glass nanopore, including its c...
Zhao-Wei Guan, De-Zhi Feng, Jin-Bo Zhu· Small Methods· 0 citations
Diabetic retinopathy (DR) is a major microvascular complication of diabetes, yet accessible molecular tools for early-stage screening remain limited. Circulating microRNAs are promising non-invasive biomarkers for DR, but their reliable quantification in serum is hindered by low abundance, matrix interference, and limi...
Tian-Shi Yang, Zi-Lin Tian, Zhao-Wei Guan et al.· Talanta: The International J...· 0 citations
DNA nanoswitches are programmable molecular devices that convert target recognition events into highly specific and controllable conformational changes. Nevertheless, their application in multiplexed analysis of low abundance miRNAs remains limited, and methodological breakthroughs are urgently needed for analysis in c...