Zheng-Ming Zhu

Enhancing the Thermostability of Sweet Protein Thaumatin II by Integrated Rational Design

Thaumatin II is a high-intensity sweet protein whose limited thermostability restricts its industrial application. Using the previously evolved high-yield Thaumatin variant M-882 as the parental scaffold, we used an integrated rational design strategy combining HotSpot Wizard, FoldX, and PyRosetta. Candidate variants...