How differences in whole wheat bread matrix properties and digestibility influence colonic fermentation remains unclear. The in vitro fecal fermentation of digested residues from four whole-wheat breads with different estimated GI (eGI) were compared. During fermentation, starch and protein contents decreased, gluten a...
AgaDcat is a promising β-agarase for the production of functional neoagarooligosaccharides (NAOSs), but its limited thermal stability and poor reusability as a free enzyme increase enzyme consumption and production cost during repeated agarose hydrolysis. In this study, β-agarase AgaDcat was immobilized on nickel-nitri...
Kaifan Qiu, Chen Wang, Xing-Fei Li et al.· Foods· 0 citations
d-Allulose 3-epimerase (DAEase) catalyzes d-fructose conversion to d-allulose, but the poor thermostability of Clostridium cellulolyticum H10 DAEase limits its industrial application. Here, we enhanced DAEase thermostability by targeting the subunit interface using PROSS-guided combinatorial engineering and spatial clu...
Kaifan Qiu, Xingfei Li, Yuxiang Bai et al.· Journal of Agricultural and...· 0 citations
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