Construction of Engineered Escherichia coli for Vanillin Production From Glucose: Pathway Module Optimization and Methyl-Donor Regulation.
Vanillin is an important flavor compound widely used in the food, fragrance, and pharmaceutical industries. Current biotransformation processes from ferulic acid or eugenol are limited by high substrate cost and low carbon efficiency, motivating de novo biosynthesis from glucose. This study employed Escherichia coli as...