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Development and evaluation of a single-tube RT-ERA-CRISPR/Cas12a assay for simultaneous detection of enterovirus G and recombinant strains with papain-like cysteine protease gene insertion

Dan-Feng Lu Zhan-Hong Li Pei-Yao Zhu Zhen-Xing Zhang Peng Liu Li Meng Xiu-Tao Sun Qi Yang Jian-Ling Song Zhuo-Ran Li
Sep 2026 · Veterinary research communications · Vol 50 · 0 citations · 46 references
Medicine

TL;DR

A single-tube reverse transcription-enzymatic recombinase amplification coupled with CRISPR/Cas12a (RT-ERA-CRISPR/Cas12a) dual-readout assay was established to concurrently detect pan-EV-G via conserved RNA-dependent RNA polymerase 3D-encoding gene and screen pathogenic PLCP-carrying recombinant strains.

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