Integrated Genomic, Transcriptomic and Metabolomic Analyses Identify Key Genetic Determinants and Regulatory Networks for Salinity Tolerance in Macrobrachium rosenbergii
Collectively, the NY strain achieves strong salinity tolerance by integrating genetic variation, transcriptional reprogramming and metabolic remodeling, synergistically maintaining ion homeostasis, osmotic balance and energy supply.
The characterization of ShNDK and the elucidation of cultivar-specific synergistic crosstalk provide a crucial mechanistic foundation and promising genetic targets for developing sugarcane cultivars with heritable, broad-spectrum resistance.
Mingjie You, Hui Li, Yan Xie et al.· Frontiers in Plant Science· 0 citations
Background/Objectives: This study aims to reveal the physiological and molecular regulatory mechanisms of the genuine medicinal herb I. suzhouensis K. F. Zhai, Z. B. Han & S. B. Zhou (Wangzaozi) of Anhui province in response to drought stress, and clarify the regulatory patterns of drought adversity on the accumulation of its medicinal active ingredients. Methods: Mild natural drought treatment was applied to I. suzhouensis. Combined with Illumina high-throughput transcriptome sequencing and HPLC-MS/MS targeted metabolomics detection, this study jointly deciphered the dynamic changes in gene expression and metabolite accumulation of I. suzhouensis under drought. Key drought-responsive metabolic pathways, core regulatory genes and marker metabolites were screened. Results: A total of 56,823 high-quality unigenes were obtained via transcriptome sequencing, among which 23,580 differentially expressed genes (DEGs) were identified. Functional enrichment analysis revealed that DEGs were predominantly enriched in pathways, including plant hormone signal transduction, phenylpropanoid biosynthesis, flavonoid biosynthesis and photosynthesis. A total of 4171 metabolites were qualitatively and quantitatively characterized via metabolomics, and 1632 differentially expressed metabolites (DEMs) were screened, mainly enriched in phenylpropanoid biosynthesis, tyrosine metabolism, flavone and flavonol biosynthesis pathways. Physiological measurements of antioxidant indices demonstrated that the activities of SOD and POD increased by approximately 2-fold, while PAL activity rose by 1.55-fold, and chlorophyll content decreased significantly. Multi-omics joint analysis indicated that mild drought stress modulates the expression of genes involved in phenylpropanoid, flavonoid and diterpenoid biosynthetic pathways, alters antioxidant enzyme activities, and coordinately regulates the formation of drought tolerance and the accumulation of bioactive compounds in I. suzhouensis. Conclusions: This study systematically elucidates the drought response mechanism of I. suzhouensis cultivated in northern Anhui province. It provides theoretical evidence and candidate core responsive gene resources for standardized cultivation of I. suzhouensis and precise regulation of medicinal quality in drought-prone production areas.
Results demonstrate that salinity tolerance in rice is associated with coordinated transcriptional and metabolic reprogramming that supports oxidative stress mitigation and adaptive stress responses.
Chander Kant Chaudhary, P. Guttula, Kirti Agrawal et al.· bioRxiv· 0 citations
First comprehensive characterization of the RLCK gene family in sugarcane is presented, elucidating its evolutionary features, expression dynamics and functional roles, and providing compelling evidence that ScRLCK53 modulates salt tolerance through activation of the JA signaling pathway.
Shichao Wang, Pingping Lin, Deng Wu et al.· Plant physiology and biochem...· 0 citations
Combined stress more strongly inhibited plant height, stem diameter, fresh weight, net photosynthetic rate, and transpiration rate than single stresses, and insights into alfalfa adaptation to multiple abiotic stresses are provided.
Lihe Su, Yongcheng Chen, Xudong Zhang et al.· Journal of Agricultural and...· 0 citations
NAC proteins are a large family of plant transcription factors that play a key role in growth, development and responses to abiotic stress (drought, salinity). Various genomic studies across several species have identified NAC genes with specific responses to cold, drought and salinity. This study conducts a comprehensive genomic and transcriptomic analysis of FaNAC genes in strawberry (Fragaria × ananassa) to elucidate their role in response to drought and salinity. Using RNA-seq and validation by RT-qPCR, thirty-five FaNAC genes were identified that showed differential expression under both stress conditions. Their expression profiles were highly specific to tissue and stimulus type: some were induced in leaves and roots under both conditions, whilst others showed restricted induction. This suggests a specialised regulatory network rather than a uniform response. Phylogenetically, the FaNAC genes showed high homology with Arabidopsis orthologues and conserved collinearity between subgenomes. The promoters contained cis elements associated with hormones and stress, particularly ABA and MeJA. FaNAC6 was selected for its strong induction in leaves and roots in response to drought and salinity, as well as under oxidative stress and ABA. Its overexpression in Nicotiana benthamiana increased stress tolerance, improving photosynthesis and water-use efficiency, and was associated with the upregulation of genes involved in photosystems, electron transport and carbon fixation. Overall, FaNAC6 emerges as a promising candidate for further evaluation in strawberry breeding strategies to improve drought and salinity resilience.
Facundo Spadoni-Revol, M. D. Moreno-Recio, Sara Aguado-Delgado et al.· International Journal of Mol...· 0 citations