Whole-genome sequence analysis of penitrem A-producing Penicillium crustosum isolated from pears: secondary metabolite biosynthesis

The genome, transcriptome and metabolome of Aspergillus welwitschiae Ocstreb1 from wild halophytic rice, Oryza coarctata, indicates its distinctiveness.

An integrated omics study provides foundational insights into the endophytic potential and genomic distinctiveness of AwOcstreb1, isolated from halophytic rice, and opens new avenues for exploring A. welwitschiae for sustainable agriculture and fungal biology.

Decoding the genome of the basidiomycetous yeast Vishniacozyma victoriae D19: a promising fungal model for biotechnology.

A high-quality genome assembly and an in-depth genome analysis of V. victoriae strain D19 are presented, establishing a valuable foundation for future functional studies and providing keys for developing a new chassis for potential industrial applications.

Complete genome sequence and mass spectrometry assist mining siderophore turnerbactin analogs in a non-model Pseudoduganella strain with biocontrol potential

Findings indicate that Pseudoduganella sp.

Comparative Whole-Genome Sequence Analysis of Alternaria alternata KACC 411286 That Produces Alternariol-Derived Toxins and Its Secondary Metabolite Biosynthetic Potential

Alternaria alternata can produce alternariol (AOH), alternariol monomethyl ether (AME), altenusin (ALN), and altenuene (ALT) on fruits and vegetables. Much is unknown about the biosynthetic gene clusters (BGCs) of secondary metabolites (SMs) including ALT in A. alternata isolated from strawberries. In the current study, we sequenced the whole genome of AOH- and AME-producing A. alternata KACC 411286 isolated from strawberry jam and carried out comparative analyses of the ALT BGC in its genome with those of other fungal strains after verification of its production of ALN and ALT. Our data showed that the assembled genome of A. alternata KACC 411286 is 34.2 Mb in size with 10 chromosomes. Gene Ontology analysis showed that genes involved in RNA transcription and protein synthesis and turnover are enriched in the genome of A. alternata KACC 411286. We identified a total of 40 SM BGCs, including the ALT BGC, in A. alternata KACC 411286. The comparative analysis showed that ALT BGCs are highly conserved between two A. alternata (KACC 411286 and ATCC 66981) and A. arborescens EGS 39–128. The functional conservation analyses of all six ALT biosynthetic genes also revealed that each gene in A. alternata KACC 411286 shares high amino acid and DNA sequence identity (above 78% identity) with its corresponding gene in four other Alternaria spp. except pksI in A. arborescens EGS 39–128 (55% identity at both the protein and DNA levels) and pksI in A. tenuissima BMP 0304 (53% at the DNA level). Our findings could provide a molecular basis for understanding the biosynthetic mechanisms of SMs, including ALT, in A. alternata KACC 411286 to reduce the contamination of fruits with multiple mycotoxins.

Comparative genomic analysis of hemicellulose-degrading potential in bacterial isolates from the anterior intestine of Eisenia andrei (Bouché, 1972)

Bioprospection of Mammaliicoccus sciuri Strain TRQ48 as a Plant-Growth-Promoting Bacteria Associated with Wheat (Triticum turgidum L. subsp. durum) in the Yaqui Valley, Mexico

Bioprospecting of plant growth-promoting bacteria enables the identification of beneficial microbial resources with significant potential for sustainable agricultural applications. In this context, strain TRQ48 was isolated from a commercial field of wheat (Triticum turgidum L. subsp. durum) located in the Yaqui Valley, Mexico, with the aim of exploring its plant growth-promoting potential. The draft genome sequence presented a genomic size of 2,777,016 bp, 32.5% G + C content, 665,763 bp N50, 2 L50, and 19 contigs. Taxonomic affiliation demonstrated that strain TRQ48 belonged to Mammaliicoccus sciuri. Genome annotation identified 2756 coding DNA sequences (CDS) distributed into 259 subsystems, highlighting CDS associated with iron acquisition and metabolism, stress response, and virulence, disease, and defense, among others. Metabolic assays reflect the strain’s capacity to produce siderophores and auxins, relating these positive traits to significantly (p ≤ 0.05) promote growth of wheat shoot length (7.09%) and root and shoot dry weight (75% and 18.43%) compared to uninoculated wheat plants. Biosafety testing indicated that the strain is susceptible to commonly used antibiotics and lacks clinically relevant resistance profiles, matching genomic analysis, which did not reveal any critical virulence factors. Thus, although the presented results show that M. sciuri TRQ48 is a promising beneficial biosafe strain, further studies are still needed to evaluate its performance under agro-ecosystems at commercial levels.