Aug 2026· Food Chemistry· Vol 526, pp.
150778
· 0 citations· 45 references
Medicine
TL;DR
This study is the first to reveal the multienzyme mechanism of OTA degradation in the genus Pediococcus, providing a theoretical basis and genetic resources for probiotic- and enzyme-based bioremediation technologies.
Abstract
This study aimed to develop efficient and environmentally friendly detoxification strategies for ochratoxin A (OTA). Pediococcus acidilactici NX8, isolated from traditional fermented dairy products, completely degraded 1 μg/mL OTA within 24 h. By integrating degradation pathway analysis and multi-omics approaches, the molecular mechanism underlying OTA degradation was elucidated, and three novel OTA-hydrolyzing enzymes (XlyAB, AmiE, and Pbp4b) were identified. The recombinant variants completely degraded 2 μg/mL OTA within 5 min and remained stable at pH 4-7 and 4-50 °C. Molecular simulations revealed the specific binding mode between the enzymes and OTA at the atomic level. This study is the first to reveal the multienzyme mechanism of OTA degradation in the genus Pediococcus, providing a theoretical basis and genetic resources for probiotic- and enzyme-based bioremediation technologies. The efficient degradation of OTA in wine and feed highlights the potential of these enzymes for applications in the food and feed industries.
The accumulation of acetaminophen (APAP) in agricultural soils and water systems poses risks to ecosystems and public health. This study characterizes Acinetobacter sp. DL27, an APAP-degrading strain with broad temperature and pH adaptability, demonstrating its bioremediation potential in soil and wastewater. Through high-resolution mass spectrometry, we identified three novel metabolic intermediates, thereby refining the bacterial APAP degradation pathway. Multiomics analysis elucidated metabolic coordination and stress-tolerance mechanisms, leading to the identification of a novel amidase (AdA). Recombinant AdA exhibited activity over 10–60 °C and pH 4.0–10.0, with a Km of 8.96 ± 1.03 μM and a kcat/Km of 9.04 μM–1s–1, indicating higher catalytic efficiency than previously reported APAP amidases. Molecular dynamics simulations and site-directed mutagenesis confirmed that a Ser161–Ser185–Lys82 triad constitutes the catalytic center driving amide cleavage. These findings provide mechanistic insights into bacterial APAP biodegradation and highlight the practical application potential of strain DL27 and AdA.
Boxiang Kou, Yufei Yue, Lei Wang et al.· Journal of Agricultural and...· 0 citations
The neonicotinoid insecticide thiamethoxam (THX) poses ecological risks and requires efficient bioremediation strategies. We isolated a highly efficient THX-degrading strain, Paenarthrobacter nicotinovorans GY-1, from contaminated agricultural soil. Under response surface-optimized conditions, GY-1 achieved an unprecedented THX degradation rate of 2.08 mg·L-1·h-1, the highest reported for a microorganism to our knowledge. Transcriptomics, enzyme assays, and carbon-source profiling showed that strain GY-1 suppresses glycolysis and reprograms central metabolism when THX is used as the sole nitrogen source. Q-TOF MS and 1D/2D NMR analyses unequivocally determined the structures of two purified intermediates, THX-1 and THX-2, providing direct structural evidence for nitro-reduction and deimination during THX biodegradation. On the basis of these confirmed intermediates, we proposed a nitro-reductive bacterial transformation pathway. This work reveals a microbial adaptation mechanism and provides a potent biocatalyst for the eco-friendly remediation of THX-contaminated environments.
Ziqing Gao, Shuya Wang, Xiu Yang et al.· Journal of Agricultural and...· 0 citations
Findings reveal that the enhanced desilication by BM3 is associated with coordinated metabolic reprogramming and a more reactive EPS-mineral interface, providing an effective biological pretreatment for disrupting silicate matrices and improving scandium recovery from refractory tailings.
Mengqi Liu, Bo Li, Feiyan Tan et al.· Bioresource Technology· 0 citations
The metabolic cooperation between plants and their endophytic fungi represents a promising frontier in the biosynthesis of natural products. This study elucidates the contribution of the endophytic fungus Fusarium oxysporum Po18 to the production of aromatic polyketides that drive specialized metabolism in Peperomia obtusifolia. Cultivation parameters for F. oxysporum were optimized using a Central Composite Rotatable Design (CCRD), revealing that mild temperatures (28 °C) and extended incubation (9 days) maximized orsellinic acid accumulation. LC–MS/MS identified orsellinic acid as [M–H]– at m/z 167.0356, with the diagnostic fragment ion m/z 122.8924, and quantified by HPLC–DAD, achieving a concentration of 132 μg/mL under optimized conditions. Comparative metabolomic analysis and molecular networking (GNPS) revealed related fungal metabolites, including lecanoric acid, 6-methylsalicylic acid, and citrinin, all derived from the fungal polyketide synthase (PKS) pathway. These metabolites are proposed to act as biosynthetic precursors for chroman and benzopyran derivatives previously reported in P. obtusifolia. The results provide the first experimental evidence of a biosynthetic partnership between Fusarium and Peperomia, in which the endophyte may supply aromatic scaffolds that could subsequently undergo downstream modifications in the host plant. This study expands the understanding of fungal–plant metabolic interactions and highlights F. oxysporum as a sustainable biotechnological source of aromatic polyketides with potential applications in natural product chemistry and biocatalysis.
Wellington Gomes de Lima, A. D. A. Morandim-Giannetti, João Luiz Bronzel Junior et al.· ACS Omega· 0 citations
A spore-free, high-yield, scalable production platform for oosporein was established, highlighting the potential of rare, protected fungal species as sources for valuable enzymes and bioactive secondary metabolites for efficient microbial biomanufacturing systems.
Niklas Broel, F. V. Wengner, J. Stein et al.· Journal of Agricultural and...· 0 citations
It is demonstrated that culture optimization is an efficient biotechnological strategy to improve 3O-methylfunicone production and support the further investigation of this compound as a scaffold for anti-H.
M. Marques, Dalila N. Loose, Crislaine S. Lima et al.· Archives of Microbiology· 1 citation
