Genomic and pan-genomic analyses of Bacillus subtilis B13 provide insights into biosynthetic potential and genetic traits associated with environmental adaptation
Insight is provided into genomic features related to the biosynthetic potential, genomic plasticity, and safety profile of B13, and suggest putative determinants of environmental adaptation, while reflecting pan-genome diversity in strain-specific traits.
Understanding of the metabolic capabilities and genomic landscape of the P. fluorescens species is enhanced, providing a foundation for natural product discovery using bioinformatic approaches.
Sajid Iqbal, Farida Begum· Discover Genetics and Evolut...· 0 citations
The complete genomic sequence and annotation information of Bacillus velezensis strain BV2007 was obtained by using PacBio Revio platform and Illumina sequencing technologies and provided a basis for further understanding of the biocontrol mechanism of B. velezensis strains.
Yinuo Liu, Tianhao Fu, Xiaohong Luo et al.· PhytoFrontiers™· 0 citations
The genome sequence provides important foundational data for further elucidating the molecular mechanisms underlying the biocontrol activity of B. velezensis strain H166 against Apple Replant Disease.
The findings suggest that B. thuringiensis JSd1 is a promising candidate for development as a biopesticide targeting insect pests in Bangladeshi agriculture, and lays the groundwork for further functional validation and field applications, contributing to sustainable pest management strategies in the region.
Md. Abdul Bari, Dipta Chandra Pal, Md. Al Muid Khan et al.· Bioinformatics and Biology I...· 0 citations
The genus
Streptomyces
is one of the richest sources of bioactive natural products; however, a substantial proportion of its biosynthetic gene clusters (BGCs) remain cryptic and their metabolic products are unresolved. Advances in genome mining and computational prediction now enable comprehensive exploration of this hidden biosynthetic repertoire. In this study, whole-genome sequencing and comparative genomic analyses were performed on three three newly isolated
Streptomyces
strains to evaluate their specialized metabolic potential. Genome assemblies were annotated and systematically analyzed using antiSMASH, DeepBGC, GECCO, and PRISM to identify, cross-validate, and functionally characterize BGCs while predicting their associated secondary metabolite scaffolds. Taxonomic analyses based on Average Nucleotide Identity (ANI), phylogenomics, and BLAST identified the isolates as
Streptomyces thinghirensis, Streptomyces novocaesareae
, and
Streptomyces griseorubens
. Applying the consensus framework across the three
Streptomyces
genomes yielded 43 cryptic BGCs, lacking close similarity to reference BGCs in the MIBiG database, of which 26 were classified as HIGH, 10 as MEDIUM, and 7 as LOW confidence. Notably, numerous BGCs exhibited low abundance to characterized reference clusters, indicating a high potential for previously undescribed biosynthetic pathways and novel metabolite scaffolds. Comparative analyses further revealed strain-specific biosynthetic architectures together with putative metal-responsive regulatory systems;
Fur, Zur
, and
Nur
, which were frequently associated with specialized metabolite biosynthetic loci. Collectively, these findings demonstrate the effectiveness of integrated genome-mining strategies for prioritizing cryptic biosynthetic gene clusters and highlight the remarkable biosynthetic potential of newly identified
Streptomyces
isolates as a source of novel natural products.
Nada S. Al-Theyab, Haila M. Alnassar, Mohanad A. Ibrahim et al.· Frontiers in Microbiology· 0 citations
The genomes of vineyard-derived Bacillus strains shown to inhibit Botrytis cinerea mycelial growth and spore germination through the production of cyclic lipopeptides and cell wall-degrading enzymes are profiled to emphasize the dynamic interplay between genomic conservation and flexibility within the Bacillus genus.
A. Kayode, Edwin I Wanjofu, M. Setati· Frontiers in Microbiology· 0 citations
