Designing and Engineering Enzymes for New-to-Nature Photobiocatalysis.

Discovery and engineering of enzymes for new-to-nature photobiocatalysis.

Photobiocatalysis integrates enzymatic catalysis with photochemistry, enabling challenging radical transformations with high selectivity under mild conditions. Early developments in this field were largely driven by the discovery that enzyme-bound cofactors can form photoactive charge-transfer complexes with substrates...

New-to-Nature Photoenzymatic Catalysis.

Beyond native reactivity: radical SAM enzymes as platforms for new-to-nature chemistry

Radical S-adenosylmethionine (rSAM) enzymes constitute one of the largest and most versatile enzyme superfamilies in biology, catalyzing diverse radical-mediated reactions essential for metabolism, cofactor biosynthesis, nucleic acid modification, and natural product formation. Central to their chemistry is the reducti...

Engineering and design of artificial enzymes for non-natural biocatalysis in whole cells.

Artificial enzymes represent transformative biocatalysts that overcome the inherent limitations of natural enzymes in substrate scope and activity specificity, enabling challenging new-to-nature reactions. Despite remarkable advances in designing diverse artificial enzymes through synthetic cofactor or non-canonical am...

[Enzymatic intermolecular Stetter reaction catalyzed by benzaldehyde lyase].

Molecular Design Principles for Photosystem I-Based Biohybrid Solar Fuel Catalysts.

Direct solar-to-chemical conversion offers a compelling route to clean, dispatchable energy. Photosystem I (PSI), an evolutionarily optimized light-driven oxidoreductase, can be repurposed for solar-fuel production by coupling its photochemistry to catalytic interfaces. However, the molecular determinants that govern p...