The small RNA tasiARF initiates a self-reinforcing morphogenic circuit connecting cell wall mechanics, auxin signaling, and regulated cell division to specify embryonic shoot stem cell fate, revealing how lineage-specific regulatory innovation can preserve a conserved developmental output across divergent embryonic architectures.
ERECTA signaling is identified as a local brake on embryonic stomatal cell maturation, discovering another way to push precocious stomatal cell maturation that results in a complex, partially mature cell state that provide insights into the limitations on cell embryonic cell maturation.
Yadhusankar Sasidharan, Vijay Suryavanshi, Pablo González-Suárez et al.· bioRxiv· 0 citations
Current knowledge about the molecular mechanisms of natural DNSO is reviewed, focusing on transcriptomic and physiological studies in model plants, to illustrate how understanding natural regeneration can guide the development of simplified, broadly applicable plant transformation technologies.
Hanim Kim, Lin Xu, Pil Joon Seo· Current opinion in plant bio...· 0 citations
This review provides an integrative overview of developmental PCD across vegetative and generative stages, from reproductive development and embryogenesis to vascular differentiation, aerenchyma formation, organ shaping, senescence, and abscission, and summarizes current knowledge of the molecular, cellular, and physiological mechanisms governing dPCD.
Jacek Łuc, M. Kwiatkowska, A. Słomka et al.· Journal of Experimental Bota...· 0 citations
MicroRNAs (miRNAs) are small non-coding RNA molecules essential for growth and development in eukaryotes. In plants, the master gene DICER-LIKE 1 (DCL1) catalyzes the biogenesis of miRNAs by processing double-stranded precursors that give rise to mature miRNAs. We sought to understand the function and evolution of microRNAs using Marchantia polymorpha, a model bryophyte that allows comparative approaches to infer characteristics of the ancestral land plant. We functionally characterized loss-of-function mutants of MpDCL1 a generated by means of CRISPR-Cas9-mediated genomic edition and the miR166/CLASS III HD-ZIP regulatory circuit in Marchantia polymorpha. We report that MpDCL1a is required for the biogenesis of miRNAs and uncovered a central role for miR166/Homeodomain Zipper Class III-regulated auxin synthesis in the specification of cell identity, patterning, meristem function, laminar expansion, and the development of the body in the last common ancestor of the bryophytes and vascular plants. Our findings indicate that DCL1, Class III HD-ZIP, miR166, and auxin functioned in the development of the body of the last common ancestor of extant land plants and provide a novel working framework to interrogate the basic principles of cell specification and patterning in plants.
Adolfo Aguilar-Cruz, E. Flores-Sandoval, Ye Xu et al.· New Phytologist· 0 citations
There is a dominant view that plants are shaped by the ability of their outermost cell layers to resist or yield to pressure from internal tissues. During stem development, outer tissues originate from the tunica layers of the shoot meristem, while inner tissues are produced by the rib zone (RZ), named after its distinctive pattern of transverse cell divisions. Despite over a century of studies, the interplay between inner and outer tissues in shaping the stem remains unclear. Here, we show that mutations in a subfamily of IQ domain (IQD) genes disrupt the orientation of cell divisions in the RZ and increase stem diameter in Arabidopsis. Measurements of cell geometry, growth of marked cell clones, tissue-specific expression, and subcellular localization all support the idea that these IQD proteins reduce longitudinal cell divisions in the RZ, limiting the build-up of vertical cell files in inner tissues and the concomitant radial growth of the stem. Thus, the characteristic orientation of cell divisions in the RZ is important for shaping the stem, and the genetic control of organ shape can be exerted in inner plant tissues.
Bryony Yates, Emma McKechnie-Welsh, Nico Mol et al.· Current Biology· 1 citation
It is reported that NtProRP1, an extracellular protein, localizes to the cell wall immediately after fertilization in Nicotiana tabacum, uncovering a new mechanism for NtProRP1 in regulating early embryogenesis and delivering a unique transcriptomic resource that advances understanding of extracellular signaling in plant embryogenesis.
An Luo, Ying Qiao, Siyuan Li et al.· Plant and Cell Physiology· 0 citations